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Home > Life Science > Genomics > DNA and RNA isolation > Plasmid Purification-Kits High-Speed / Fast Ion > IBI Scientific MIDI Fast-Ion Plasmid kit 25 preps IB47111

IBI Scientific MIDI Fast-Ion Plasmid kit 25 preps IB47111
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IBI Scientific MIDI Fast-Ion Plasmid kit 25 preps IB47111

PART NO: 4AJ-4661763
Price: £244.73
Qty In Stock: 4
The Midi Fast Ion Plasmid Kit (25 preps) uses pre-packed ion-exchange resin columns to purify plasmid or cosmid DNA from bacterial cultures.

The quality of the Midi Fast Ion Plasmid Kit is tested on a lot-to-lot basis by isolating plasmid DNA from a 50 ml overnight E. coli (DH5?) culture, containing pBluescript (A600>2U/ ml). More than 120 µg of plasmid DNA is quantified with a spectrophotometer. The purified plasmid (1 µg) is used in EcoRI digestion, and checked by electrophoresis.

- Sample Size: 50ml high-copy plasmid/100ml low-copy plasmid
- Operation: Gravity Flow Column
- Expectant Yield: 200µg of plasmid DNA
- Operation Time: 120 minutes or less

Specifications Midi Fast Ion Plasmid Kit
Format: Anion-Exchange
Operation: Gravity Flow
Binding Capacity: 800 µg
Sample Concentration: 50-300 ml
Fragment Size: 1-20 kb
Typical Yield: 200-800 µg
Elution Volume: 500 µl- 2 ml
Operation Time: <120 minutes

How It Works?


In this process, the modified alkaline lysis method and RNase treatment are used for creating cleared lysate with minimal genomic DNA and RNA contaminants.

Using a gravity-flow procedure, the plasmid DNA in crude lysate has been bound to the column. The contaminants can be washed off with a wash buffer. Finally, the purified DNA is eluted by a high salt buffer and then precipitated with isopropanol for desalting. The entire procedure can be completed in less than two hours. The purified plasmid DNA is suitable for transfection, sequencing reactions, PCR, and in-vitro transcription.

FAQ



What competitor’s kit is comparable?

QIAGEN Plasmid Maxi Kit or QIAGEN QIAfilter Plasmid Maxi Kit


What is the formulation for the PW buffer in the Midi plasmid kit?

This is not exact, but it is QIAGAN’s formulation for a similar kit. The formulation will work fine. 1.0 M NaCl, 50 mM MOPS, pH 7.0 15% Isopropanol.