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Home > Life Science > Cell Culture > Classical Media > RPMI 1640 Media > RPMI 1640 without L-Glutamine Bioconcept 1-41F01-I

RPMI 1640 without L-Glutamine Bioconcept 1-41F01-I
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RPMI 1640 without L-Glutamine Bioconcept 1-41F01-I

PART NO: BC-1-41F01-I
Price: £11.59
Out of Stock (Ships in approx. 1 week)
Minimum quantity of 6

RPMI 1640 was developed as a modification of McCoy's 5A medium and is based on RPMI 1630. RPMI 1640 uses a bi-carbonate buffering system and differs from most mammalian cell culture media in its typical pH 8 formulation. With or without supplemented serum or other defined proteins, RPMI 1640 represents today’s most widely applied culture medium for short as well as long term cultivation of many cell types, especially human T/B-lymphocytes (e.g. 72h PHA stimulation assay), bone marrow cells, hybridoma cells.


Inorganic Salts mg / L mg / L
Ca(NO3)2 4H2O  100
Na2HPO4 anhydrous  800
MgSO4 7H2O  100
KCl  400
NaCl  6000
NaHCO3  2000
Other Components  
4-Aminobenzoic acid  1
D(+)-Glucose  2000
L-Glutathione reduced  1
Phenol Red  5
Amino Acids  
Glycine  10
L-4-Hydroxyproline  20
L-Arginine HCl  241.9
L-Asparagine anhydrous  50
L-Aspartic acid  20
L-Cystine  50
L-Glutamic acid  20
L-Histidine  15
L-Isoleucine  50
L-Leucine  50
Amino Acids mg / L  
L-Lysine HCl  40
L-Methionine  15
L-Phenylalanine  15
L-Proline  20
L-Serine  30
L-Threonine  20
L-Tryptophan  5
L-Tyrosine  20
L-Valine  20
Vitamins  
(+)-Biotin  0.2
Choline chloride  3
D-Pantothenic acid calcium salt  0.25
Folic acid  1
myo-Inositol  35
Nicotinamide  1
Vitamin B1 HCl  1
Vitamin B2  0.2
Vitamin B6 HCl  1
Vitamin B12  0.005
To be added separately  
L-Glutamine  300