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M-199 EBS without L-Glutamine Bioconcept 1-21F01-I

PART NO: BC-1-21F01-I

Price: £11.41

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Medium 199 is a totally defined nutritive source for explant tissues and cells in cell culture. Properly supplemented with serum, Medium 199 offers broad applicability for cells of different animal species, particularly for the culture of non-transformed cells. It is also used in virology for vaccine production.

Specification

Inorganic Salts mg / L	mg / L
CaCl2 2H2O	265
Fe(NO3)3 9H2O	0.72
MgSO4 7H2O	200
KCI	400
NaCl	6800
NaH2PO4 H2O	140
NaHCO3	2200
Other Components
2-Deoxy-D-ribose	0.5
4-Aminobenzoic acid	0.05
Adenine sulfate	10
AMP H2O	0.25
ATP disodium salt H2O	1
Cholesterol	0.2
D(+)-Glucose	1000
D(-)-Ribose	0.5
Guanine HCI	0.3
Hypoxanthine	0.3
L-Glutathione reduced	0.05
Phenol Red	20
CH3COONa anhydrous	50
Thymine	0.3
Tween 80	20
Uracil	0.3
Xanthine	0.3
Amino Acids
Glycine	50
L-4-Hydroxyproline	10
L-Alanine	25
L-Arginine HCl	70
L-Aspartic acid	30
L-Cysteine HCL, H2O	0.11
L-Cystine	20
Amino Acids mg / L
L-Glutamic acid	66.82
L-Histidine HCL, H2O	21.88
L-Isoleucine	20
L-Leucine	60
L-Lysine HCl	70
L-Methionine	15
L-Phenylalanine	25
L-Proline	40
L-Serine	25
L-Threonine	30
L-Tryptophan	10
L-Tyrosine	40
L-Valine	25
Vitamins
(+)-Biotin	0.01
Vitamin K3	0.02
Choline chloride	0.5
D-Pantothenic acid calcium salt	0.01
Folic acid	0.01
myo-Inositol	0.05
Nicotinamide	0.025
Nicotinic acid	0.025
Pyridoxal HCl	0.025
Vitamin B1 HCl	0.01
Vitamin B2	0.01
Vitamin B6 HCl	0.025
Vitamin C	0.05
Vitamin D2	0.1
Vitamin E succinate	0.01
Vitamin A acetate	0.14
To be added separately
L-Glutamine	100