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DMEM Low Glucose without L-Glutamine Bioconcept 1-25F01-I

PART NO: BC-1-25F01-I

Price: £12.10

Out of Stock (Ships in approx. 1 week)

Dulbecco's MEM is the most widely used modification of BME. It contains four times the concentration of amino acids and vitamins. Non-essential amino acids and certain essential trace elements were added and the bicarbonate concentration was increased. The standard formula is with 1000 mg/L glucose, the “high glucose” variation with 4500 mg/L. Dulbecco’s MEM was originally developed for the culture of mouse embryonic cells. Today it finds a broad application for serum free culture of normal and transformed mouse and chicken cells, e.g. 1:1 with Ham's F-12.

Specification

Inorganic Salts	mg / L
CaCl2 2H2O	264
Fe(NO3)3 9H2O	0.1
MgSO4 7H2O	200
KCl	400
NaCl	6400
NaH2PO4 H2O	125
NaHCO3 3	700
Other Components
D(+)-Glucose	1000
Phenol Red	15
Pyruvic acid sodium salt	110
Amino Acids
Glycine	30
L-Arginine HCl	84
L-Cystine	48
L-Histidine HCl H2O	42
L-Isoleucine	105
L-Leucine	105
L-Lysine HCl	146
Amino Acids mg / L
L-Methionine	30
L-Phenylalanine	66
L-Serine	42
L-Threonine	95
L-Tryptophan	16
L-Tyrosine	72
L-Valine	94
Vitamins
Choline chloride	4
D-Pantothenic acid calcium salt	4
Folic acid	4
myo-Inositol	7.2
Nicotinamide	4
Vitamin B1 HCl	4
Vitamin B2	0.4
Vitamin B6 HCl	4
To be added separately
L-Glutamine	584